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KMID : 0043320180410090875
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Archives of Pharmacal Research
2018 Volume.41 No. 9 p.875 ~ p.884
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High-throughput genetic screens using CRISPR/Cas9 system
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Kweon Ji-Yeon
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Abstract
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The CRISPR/Cas9 system is a powerful tool for genome engineering, and its programmability and simplicity have enabled various types of gene manipulation such as gene disruption and transcriptional and epigenetic perturbation. Particularly, CRISPR-based pooled libraries facilitate high-throughput screening for functional regulatory elements in the human genome. In this review, we describe recent advances in CRISPR/Cas9 technology and its use in high-throughput genetic screening. We also discuss its potential for drug target discovery and current challenges of this technique in biomedical research.
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KEYWORD
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CRISPR/Cas9 system, Genome engineering, High-throughput screening, Pooled CRISPR screens
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